Friday, July 31, 2015

Mitosis

Different Phases in cell cycle:
 
Cell cycle consists of  G1 ,S, G2 and M phases:
       G1
            } Gap Phase
       G2
G1, G2 and S Phase together called as Interphase.

G1 Phase:
the first growth period of the cell cycle,, the cell grows in size and synthesizes mRNA and proteins that are required for DNA

synthesis.
S Phase:Synthesis phase ,replication takes place .S phase a number of events additional to chromosomee replication take place. Cell growth continues through S phase, as does the rate of synthesis of a number of proteins and enzymes that are involved in DNA synthesis. Once DNA replication is complete the cell contains twice its normal number of chromosomes and becomes ready to enter the phase called G2. 

G2 Phase:
the second growth period of the cell cycle, following DNA replication and preceding prophase,It synthesizes the protein that constructs the microtubules, which are responsible for separating the chromosomes in the anaphase of mitosis.
Length of cell cyle( time for each phases):
The length of the cell cycle is important because it determines how quickly an organism can multiply. For single-celled organisms, this rate determines how quickly the organism can reproduce new, independent organisms. For higher-order species the length of the cell cycle determines how long it takes to replace damaged cells. The duration of the cell cycle varies from organism to organism and from cell to cell.
Time take for cell cycle differ from species to species , generally unicellular organisms DNA is very less ,so cell cycle is faster than multicellur organisms. Sothe lengths of G1 and G2 vary in cells based on the individual cell's level of preparedness for proceeding in the cell cycle.
G1 is typically the longest phase of the cell cycle it is around 10 hrs time.
S phase takes 5 to 6 hrs
G2 Phase takes 3 to 4hrs 
that means interphase  in between 18  to 20 hrs. 


Cell Cycles

Chromosomes and chromatin structure

Cell Division 

Meiosis  

Eukaryotic Gene and Gene Expression





Mitotic Division:
Mitosis is a process of nuclear division in eukaryotic cells that occurs when a parent cell divides to produce two identical daughter cells by which the body replaces dead cells. In this process daughter cells are also diploid like parent . 
The time period between two mitotic cells is called Interphase.
Mitotic division divided into Prophase, Metaphase, Anaphase and Telophase.  Cytokinesis is the final physical cell division that follows telophase.
Interphase : it is not actual mitotic division but it is remainder for proceeding of mitotc division  .
Prophase: 
1.During prophase, the replicated pairs of chromosomes condense and compact themselves. The pairs of chromosomes that have been replicated are called sister chromatids, and they remain joined at a central point called the centromere. 
2.Nuclear membrane is disappear and centrosome which is attaches to nucleus.At prophase period centrosomes divide into two daughter centrosomes  and move towards poles . The mitotic spindle then forms between the two centrosomes, spindle fibers are made up of  microtubule which is cytoskeleton .
3.Spindle fibers attach to the centromere via the Kinetochore .Kinetochore is a protein which form around centromere.
MetaPhase:
When the centromeres of all the cell's chromosomes line up at the equator of the spindle. Metaphase is particularly useful in cytogenetics, because chromosomes can be most easily visualized at this stage.
Anaphase: 
1.The kinetochore microtubules shorten,the centromeres split separating the two members of each chromatid pair - which then move to the opposite poles of the cell.When they are separated the chromatids are called chromosomes.
2.As the chromosomes are pulled by the the microtubules, they appear to be "V"-shaped because the centromeres lead the way, dragging the trailing arms of the chromosomes towards the poles.
3.Anaphase ensures that each daughter cell receives an identical set of chromosomes.
Telophase:
1.Telophase begins after the chromosomal movement stops.
2.Chromosomes became uncoil and revert to the long, thin, thread-like chromatin form.
3. Nuclear envelope forms around each chromatin mass.
Along with telophase, the cell undergoes a separate process called cytokinesis that divides the cytoplasm of the parental cell into two daughter cells.